Testosterone Levels And Dna Fragmentation Index In Male Undergoing Intrauterine Insemination After Vitamin D3 Supplementation

Agung Sari Wijayanti; Uki Retno Budihastuti; Eriana Melinawati; Abdurahman Laqif; Fatmiyanti Fahsan

doi:10.11594/ibehs.vol13iss1pp16-22

Authors

Agung Sari Wijayanti Department of Obstetrics and Gynecology, Dr. Moewardi Hospital Surakarta, Indonesia
Uki Retno Budihastuti Department of Obstetrics and Gynecology, Dr. Moewardi Hospital Surakarta, Indonesia. 57126 https://orcid.org/0000-0003-0611-7464
Eriana Melinawati Department of Obstetrics and Gynecology, Dr. Moewardi Hospital Surakarta, Indonesia. 57126 https://orcid.org/0000-0003-1530-7446
Abdurahman Laqif Department of Obstetrics and Gynecology, Dr. Moewardi Hospital Surakarta, Indonesia. 57126 https://orcid.org/0000-0002-6642-5701
Fatmiyanti Fahsan Department of Obstetrics and Gynecology, Faculty of Medicine, Sebelas Maret University, Surakarta, Indonesia. 57126

DOI:

https://doi.org/10.11594/ibehs.vol13iss1pp16-22

Keywords:

Blood Testosterone, DFI, Infertility, Vitamin D, , Intrauterine Insemination

Abstract

Nutrition is one of the factors that contribute to male infertility. Vitamin D3 are known to affect spermatozoa and usually served in fertility supplementation in doses of 400 and 1000 IU. Low levels of vitamin D3 have been linked to decreased sperm quality and quantity. This research would like to know the effect of Vitamin D3 to the DNA Fragmentation Index (DFI) and Testosterone blood level in men undergoing intrauterine insemination (IUI). This was an analytical observational study with two groups and a pretest-posttest design. Seventeen men aged 20 to 45 who were undergoing the IUI program and did not have diabetes or hypertension are participate in this study. Sperm DFI was determined by using an ELISA method with a DNA Kit. Blood testosterone levels in ng/mL units was measure using ELISA. IBM-SPSS version 22 was used to perform univariate and bivariate analysis on the collected data. The blood level of Vitamin D3 was significantly increased after the intervention of six week Vitamin D3. Meanwhile, this study shows there are no differences in the level of blood testosterone before and after treatment. The DFI was significantly increased in the 400 IU groups.

References

Amrein, K. et al. (2020) ‘Vitamin D deficiency 2.0: an update on the current status worldwide’, European Journal of Clinical Nutrition, 74(11), p. 1498. doi: 10.1038/S41430-020-0558-Y.

Calagna, G. et al. (2022) ‘Vitamin D and Male Reproduction: Updated Evidence Based on Literature Review’, Nutrients, 14(16), pp. 1–10. doi: 10.3390/nu14163278.

Chen, C. et al. (2019) ‘Causal Link Between Vitamin D and Total Testosterone in Men: A Mendelian Randomization Analysis’, The Journal of clinical endocrinology and metabolism, 104(8), pp. 3148–3156. doi: 10.1210/JC.2018-01874.

Damas-Fuentes, M. et al. (2022) ‘25-hydroxyvitamin D and testosterone levels association through body mass index: A cross-sectional study of young men with obesity’, Frontiers in Endocrinology, 13. doi: 10.3389/fendo.2022.960222.

Deng, X.-L. et al. (2014) ‘[Efficacy and safety of vitamin D in the treatment of idiopathic oligoasthenozoospermia].’, Zhonghua nan ke xue = National journal of andrology, 20(12), pp. 1082–5.

Derakhshan, Maryam et al. (2020) ‘The association between serum vitamin D level and sperm parameters; A pilot study in a subset of Iranian infertile males’, Immunopathologia Persa, 6(2), pp. e30–e30. doi: 10.34172/IPP.2020.30.

Karaplis, A. C. et al. (2011) ‘Niveles de vitamina d y respuesta a dosis diarias de 400 iu de vitamina d3 y semanales de 70 mg de alendronato en hombres y mujeres canadienses con osteoporosis’, Annals of Pharmacotherapy, 45(5), pp. 561–568. doi: 10.1345/aph.1P439.

Lerchbaum, E. et al. (2019) ‘Effects of vitamin D supplementation on androgens in men with low testosterone levels: a randomized controlled trial’, European Journal of Nutrition, 58(8), pp. 3135–3146. doi: 10.1007/s00394-018-1858-z.

Leslie, S. W., Soon-Sutton, T. L. and Khan, M. A. (2023) ‘Male Infertility’, StatPearls.

Maghsoumi-Norouzabad, L. et al. (2021) ‘The effects of Vitamin D3 supplementation on Spermatogram and endocrine factors in asthenozoospermia infertile men: a randomized, triple blind, placebo-controlled clinical trial’, Reproductive Biology and Endocrinology, 19(1), p. 102. doi: 10.1186/s12958-021-00789-y.

Özçelik, S. et al. (2018) ‘Distribution of childhood skin diseases according to age and gender, a single institution experience’, Turkish Archives of Pediatrics/Türk Pediatri Arşivi, 53(2), p. 105. doi: 10.5152/TURKPEDIATRIARS.2018.6431.

Wadhwa, L. et al. (2020) ‘Impact of Vitamin D Supplementation on Semen Quality in Vitamin D-Deficient Infertile Males with Oligoasthenozoospermia’, Journal of Obstetrics and Gynaecology of India, 70(1), p. 44. doi: 10.1007/S13224-019-01251-1.